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My Cart> Biochemical Reagents > Other Biochemical Reagents > Erythrina Cristagalli Lectin (ECL, ECA), Biotinylated
Storage：Short-term storage at 2-8℃;long-term storage at -20℃
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| Storage | Short-term storage at 2-8℃;long-term storage at -20℃ |
| Concentration | 5mg/ml |
| Unit | piece |
| Specification | 1mL |
Ecl-bound carbohydrate structures are commonly found in membrane and serum glycoproteins of mammalian origin. This structural sialic acid substitution appears to prevent lectins from binding. This specificity provides an opportunity to isolate or isolate mammalian glycoproteins using agarose bound ECL. It has been reported that the lectin can be used to isolate human natural killer (NK) cells using negative selection screening techniques. NK cells appear to lack the surface sugar structure needed to bind ECL, and unlike other monocytes, it does not attach to a petri dish coated with ECL. Since this process involves negative selection screening techniques, a high recovery rate of active NK cells can be obtained. Adherent cells can also be recovered by culture in galactose or lactose. Biotinylated Erythrina cristagalli lectin has the right amount of biotin to provide the best staining properties for this lectin. The conjugated compound contains essentially no unconjugated biotin and is preserved with sodium azide.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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