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Strip size:
25,49,72,100,130,160,200,250,300 kDa
Buffer:
62.5 mM Tris-H3PO4 (pH 7.5), 2 mM EDTA, 2% (W/V) SDS, 33% (W/V) Glycerol, 10 mM DTT
Product Introduction:
Color prestain protein Marker (25-300 kD, tricolor) is a mixture of three color prestain proteins. The product is prepared by premixing 9 kinds of high purity recombinant proteins of different molecular weight sizes, covering the molecular weight range of 25-300 kDa (25,49,72,100,130,160,200,250,300 kDa), of which 25 kDa is a green band, 72 kDa is an orange band. The remaining 7 proteins are blue bands.
This product is used as the protein molecular weight standard for Western Blot or SDS-PAGE to indicate the electrophoretic protein separation of SDS-PAGE and to evaluate the membrane transfer effect of Western Blot. The migration of this product in different gel concentrations and different electrophoresis buffers is shown in the figure on the right. The product is pre-mixed with loading buffers and can be loaded directly into the protein gel pores without heating, diluting or adding reducing agents.
