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CAS:9024-52-6
Appearance:Lyophilised powder
Storage:2-8℃
Product Introduction
Aldolase is present in all animal and plant tissues, as well as in most microorganisms. Class I aldehydases are found in animal and higher plant tissues and are characterized by the absence of bivalent metal cofactors and form ketamine Schiff base intermediates with the substrate dihydroxyacetone phosphate. Class II aldolases exist only in prokaryotes and lower eukaryotes and require bivalent metal cofactors. There are three types of Class I aldolases: Type A (the main form) is found in muscles; Liver and kidney type B; And type C in the brain (plus some Type A).
Application
Chemical enzyme synthesis; Structure-function study; Organic synthesis; Quantitative analysis of D-fructose-1, 6-diphosphate; Determination of metabolites in coupling reactions; Molecular weight labeling column and gel
Precautions
The enzyme is irreversibly denatured at pH below 4.5
International System Classification Number: EC: 4.1.2.13
Optimum pH: 6.9-8.8
Isoelectric point: 8.47
Inhibitors: Heavy metals, especially Cu2+, Zn2+ and Ag+; Phosphohydroxynaphthalaldehyde derivatives; Phosphorylated aromatic compounds; D-erythritone 1-phosphate; Phosphatidylserine liposomes; Phosphoric acid and adenine nucleotides
Definition of unit: At 25 ℃, pH 7.5, measured with hydrazine/glyceraldehyde 3-phosphate, A240 increased by 1.0 per minute
