Product Introduction

Aspergillus oryzae ribonuclease T2 is a member of the ribonuclease T2 family and is widely present in various microbial, plant and animal species. In contrast to Aspergillus oryzae ribonuclease T1, which is a completely specific guanylate endonuclease, all ribonuclease T2-like enzymes are essentially non-base specific. However, RNase T2 endonuclease enzymes from different species can exhibit a slight base preference. Fungal enzymes, including Aspergillus oryzae RNaseT2, show a slight base preference, in the following order :A> G> C, RNaseT2 at 3 ' of a base; 5 ' of phosphoric acid residues and adjacent nucleotides; The -OH residues are cut between them to form a 2 ' , 3 ' -cyclic phosphoric acid intermediates are then generated with 3 ' Oligonucleotides of phosphoric acid residues.

Application

Used on RNA; End analysis and RNase protection test

Precautions

EDTA stimulates activity, especially in the presence of bivalent cations

At 37 ℃ and pH 4.5, one unit increases the absorbance at 260 nm by 1.0 in 15 minutes

Unit definition: at 37 ℃ and pH 4.5, one unit increases the absorbance at 260 nm by 1.0 in 15 minutes

International System Classification Number: EC: 4.6.1.19

Optimal pH: 4.5

Isoelectric point: 5

Inhibitor: Strongly inhibited by Cu2+, Zn2+ and Hg2+; Ca2+, Mg2+ and heparin (to a lesser extent) mononucleotide and RNase T2 digestive products (competitive)