Ribonuclease

Product Introduction

Aspergillus oryzae ribonuclease T2 is a member of the ribonuclease T2 family and is widely present in various microbial, plant and animal species. In contrast to Aspergillus oryzae ribonuclease T1, which is a completely specific guanylate endonuclease, all ribonuclease T2-like enzymes are essentially non-base specific. However, RNase T2 endonuclease enzymes from different species can exhibit a slight base preference. Fungal enzymes, including Aspergillus oryzae RNaseT2, show a slight base preference, in the following order :A> G> C, RNaseT2 at 3 ' of a base; 5 ' of phosphoric acid residues and adjacent nucleotides; The -OH residues are cut between them to form a 2 ' , 3 ' -cyclic phosphoric acid intermediates are then generated with 3 ' Oligonucleotides of phosphoric acid residues.

Application

Used on RNA; End analysis and RNase protection test

Precautions

EDTA stimulates activity, especially in the presence of bivalent cations

At 37 ℃ and pH 4.5, one unit increases the absorbance at 260 nm by 1.0 in 15 minutes

Unit definition: at 37 ℃ and pH 4.5, one unit increases the absorbance at 260 nm by 1.0 in 15 minutes

International System Classification Number: EC: 4.6.1.19

Optimal pH: 4.5

Isoelectric point: 5

Inhibitor: Strongly inhibited by Cu2+, Zn2+ and Hg2+; Ca2+, Mg2+ and heparin (to a lesser extent) mononucleotide and RNase T2 digestive products (competitive)

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.