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重组脱氧核糖核酸酶Ⅰ(Animal Free/AF)

CAS:9003-98-9

Appearance:Solution

Storage:Store at -20℃,2 years

重组脱氧核糖核酸酶Ⅰ(Animal Free/AF)
Cat.No:
D8073
Brand:
Solarbio

Product Introduction

Bovine pancreatic deoxyribonuclease I is regenerated in the yeast Pichia pastoris to reduce levels of contaminated RNase and eliminate potential pathogens associated with animal-based materials. Bovine pancreas is A rich source of RNase A, commonly found in many commercial DNase preparations. The recombinant production of DNase I in organisms with much lower endogenous RNase levels greatly facilitates the purification of enzymes that are undetectable at RNase levels. The production and separation process of recombinant DNase I is completely free of animal components, thus eliminating the possibility of introducing animal-derived pathogens into bioprocess procedures.

International System Classification Number :EC: 3.1.21.1

Unit definition

At a pH of 5.0, when 1 unit is used for highly polymerized DNA at 25℃, the absorbance at 260nm increases by 0.001/min/ml

Application

Genomic DNA is removed from the RNA preparation prior to RT-PCR

Degradation of DNA templates after a transcriptional reaction, removing unwanted DNA from the sample prior to Northern imprinting

DNA removal in biopharmaceutical and bioprocess processes

Precautions

Avoid repeated freezing and thawing.

Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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