Deoxyribonuclease I

Product Introduction

Bovine pancreatic deoxyribonuclease is an endonuclease that preferentially splits phosphodiester bonds adjacent to pyrimidine nucleotides to produce 5' Phosphoric acid terminal polynucleotide, at 3' The site has a free hydroxyl group. Deoxyribonucnase I is secreted by exocrine glands and is highest in the pancreas and parotid glands. It is also found in low amounts in other tissues. This product removes proteases and RNA enzymes and is suitable for RNA recycling or for research where RNA and protein integrity is required. DNase is known to be involved in cell apoptosis and is thought to play a role in the regulation of actin polymerization in cells. In addition to its applications in molecular biology, DNase I is also used to treat cystic fibrosis and systemic lupus erythematosus.

International System Classification Number :EC: 3.1.21.1

Optimum pH:7.8

Isoelectric point :5.08

Inhibitors: 2-mercaptoethanol 2-nitro-5-thiocyanobenzoate actin EGTA sodium dodecyl sulfate calf spleen inhibitor protein carbodiimide and cholesterol sulfate iodoacetate

Unit definition: When pH is 5.0 and 1 unit is used for highly polymerized DNA at 25 ℃, the absorbance at 260nm increases by 0.001/min/ml

Application

DNA removal in primary cell isolation

DNA removal in bioprocessing

Genomic DNA is removed in RT-PCR

In vitro transcription

Protein and nucleic acid purple diplomatic link

radiolabelling

Unit definition

At a pH of 5.0, when 1 unit is used for highly polymerized DNA at 25℃, the absorbance at 260nm increases by 0.001/min/ml

Precautions:Avoid repeated freezing and thawing

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.