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Your Position: Home > Biochemical Reagents > Enzymes & Coenzymes > Clostripain
Clostripain

CAS:9028-00-6

Appearance:Lyophilised powder

Storage:Store at 2-8℃

Clostripain
Cat.No：
C3170
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
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Product Information Related Products Related Paper COA Technical Q&A

Product Introduction

Clostripain (Endoproteinase-Arg-C) is a collagenase-related double-chain protease isolated from Clostridium histolytica. It is highly specific to the carboxyl peptide bonds of arginine. Clostripain needs sulfhydryl; It is activated by dithithreitol, cysteine, or other agents containing sulfhydryl groups. The presence of calcium ions is essential

Application

Peptide map; Sequence analysis; Cell segregation; Hydrolysis/condensation of amide bonds; Peptide synthesis

Precautions

It is grown in a completely animal-free medium and is designed for bioprocessing applications where the introduction of pathogens of animal origin must be prevented.

International System Classification Number: EC: 3.4.22.8

Optimal pH: 7.4-7.8

Isoelectric point: 4.8-4.9

Inhibitor: EDTA; Oxidizing agent; Sulfhydryl reagents (such as TLCK); Co2+, Cu2+, Cd2+, and heavy metal ions; Citrate, borate and Tris anion were partially inhibited

Unit definition: Hydrolyzed 1 micromole of N-benzoyl-L-arginine ethyl ester 1 unit per minute in the presence of dithithreitol at 25 ℃ and pH 7.6.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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