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CAS：9028-00-6
Molecular Weight：53kDa
Appearance：Lyophilised powder
Storage：Store at 2-8℃
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| Name | Clostripain |
| CAS | 9028-00-6 |
| Molecular Weight | 53kDa |
| Storage | Store at 2-8℃ |
| Appearance | Lyophilised powder |
| Source | Clostridium histolyticum |
| Enzyme Activity/Titer | ≥50 units per mg dry weight |
| Unit | piece |
| Specification | 1mg 5mg |
Product Introduction
Clostripain (Endoproteinase-Arg-C) is a collagenase-related double-chain protease isolated from Clostridium histolytica. It is highly specific to the carboxyl peptide bonds of arginine. Clostripain needs sulfhydryl; It is activated by dithithreitol, cysteine, or other agents containing sulfhydryl groups. The presence of calcium ions is essential
Application
Peptide map; Sequence analysis; Cell segregation; Hydrolysis/condensation of amide bonds; Peptide synthesis
Precautions
It is grown in a completely animal-free medium and is designed for bioprocessing applications where the introduction of pathogens of animal origin must be prevented.
International System Classification Number: EC: 3.4.22.8
Optimal pH: 7.4-7.8
Isoelectric point: 4.8-4.9
Inhibitor: EDTA; Oxidizing agent; Sulfhydryl reagents (such as TLCK); Co2+, Cu2+, Cd2+, and heavy metal ions; Citrate, borate and Tris anion were partially inhibited
Unit definition: Hydrolyzed 1 micromole of N-benzoyl-L-arginine ethyl ester 1 unit per minute in the presence of dithithreitol at 25 ℃ and pH 7.6.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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