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CAS:37278-25-4
Appearance:Liquid
Storage:Store at -20℃，6 months
Product Introduction
Nuclease S1 is isolated from certain neurosporus and Aspergillus and exists as a monomer. It hydrolyzes the ends and internal phosphodiester bonds of single-stranded DNA and RNA.
Application
Eliminate unannealed polynucleotide tails and hairpin rings in DNA-RNA or DNA-DNA double strands
Precautions
Zinc ion and calcium ion can activate it.
When diluted with water to ≥6000U/mL, it can be frozen for 6 months;
The diluted solution can be stabilized by adding 0.1% albumin and 10% glycerin.
Unit definition: One unit can hydrolyze 1ug of denatured calf thymus DNA per minute at 37 ℃ and pH 4.6
International System Classification Number: EC: 3.1.30.1
Optimal pH: 4.0-4.6
Inhibitors: EDTA, Citrate, SDS in high concentrations
Product Introduction
