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CAS：37278-25-4
Molecular Weight：34kDa
Appearance：Liquid
Storage：Store at -20℃，6 months
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| Name | Nuclease |
| CAS | 37278-25-4 |
| Molecular Weight | 34kDa |
| Storage | Store at -20℃，6 months |
| Appearance | Liquid |
| Source | Aspergillus oryzae |
| Unit | piece |
| Specification | 10ku 50ku |
Product Introduction
Nuclease S1 is isolated from certain neurosporus and Aspergillus and exists as a monomer. It hydrolyzes the ends and internal phosphodiester bonds of single-stranded DNA and RNA.
Application
Eliminate unannealed polynucleotide tails and hairpin rings in DNA-RNA or DNA-DNA double strands
Precautions
Zinc ion and calcium ion can activate it.
When diluted with water to ≥6000U/mL, it can be frozen for 6 months;
The diluted solution can be stabilized by adding 0.1% albumin and 10% glycerin.
Unit definition: One unit can hydrolyze 1ug of denatured calf thymus DNA per minute at 37 ℃ and pH 4.6
International System Classification Number: EC: 3.1.30.1
Optimal pH: 4.0-4.6
Inhibitors: EDTA, Citrate, SDS in high concentrations
Product Introduction
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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