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Your Position: Home > Biochemical Reagents > Enzymes & Coenzymes & Inhibitors > Diamine Oxidase from Porcine Kidney
Diamine Oxidase from Porcine Kidney

CAS:9001-53-0

Appearance:Solid

Storage:Store at -20℃

Diamine Oxidase from Porcine Kidney
Cat.No：
D3830
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
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Product Information Related Products Related Paper COA Technical Q&A

Product Introduction

Porcine kidney derived diamine oxidase is a homodimer composed of two identical subunits with a molecular weight of 87kDa, each containing a pyridoxal phosphate molecule and a copper atom. The molecular weight of the enzyme is 170kDa. The enzyme is a glycoprotein containing 5% hexose, 3.3% glucosamine, 2.6% n-acetylglucosamine, and 0.25% n-acetylneuraminic acid. The enzyme has A high affinity for canavalin A. The optimum pH for cadaverine and histamine as substrates is 6.3-7.4.

Application

Diamine oxidase from pig kidneys has been used to investigate luminescent based tests for the determination of ornithine decarboxylase activity. Diamine oxidase from pig kidney was also used to study the n-linked oligosaccharide structure in diamine oxidase.

Diamine oxidase derived from pig kidney has been used to prepare histamine biosensors.

Precautions

Diamine oxidase catalyzes the oxidation of monoamine, diamine and histamine to aldehydes, ammonia and hydrogen peroxide. This enzyme belongs to the copper containing amine oxidase, which is a key enzyme in nitrogen metabolism. Diamine oxidase is inhibited by diethyldithiocarbamate, phenylhydrazine, semicarbazone, cyanide and isonicotinate hydrazine.

International System Classification Number: EC: 1.4.3.22

Unit definition: At pH 7.2 and 37 ℃, 1 unit per hour will oxidize 1.0 μmol putrescine

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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