Plasma Amine Oxidase

Product Introduction

There are two types of amine oxidase: pyridoxal-containing and cupr-containing enzymes to which plasma amine oxidase belongs and FAD-containing amino oxidase. Natural substrates include catecholamines, tryptamine derivatives, and other physiologically active amines. Plasma amine oxidase is used for studies requiring nitrogen group transfer. The molecule consists of two identical polypeptide chains. Each molecule has two pyridoxal phosphate and two Cu+ atoms. Bovine plasma amine oxidase is inhibited by many carboxyl reagents such as copper chelating agent, copper ketone, hydroxylamine and cyanide. Benzoic acid and benzyl alcohol are non-competitive inhibitors (KI 30 and 34mM, respectively)

Application

Plasma amine oxidase is used for studies requiring nitrogen group transfer.

Precautions

The e main physiological amines that are oxidized are spermidine and spermidine, and also have some activity against benzylamine, sulfanilamide, furfuramine and monofatty amine. It is only slightly active against tyramine, and has no activity against tryptamine, epinephrine, serotonin, or agmatine

International System Classification Number: EC: 1.4.3.21

Optimal pH: spermidine is 6.2 and spermidine is 7.2. Other amines may have other optimal pH values

Isoelectric point: 5.63

Inhibitors: Copper chelating agents, many carboxyl reagents such as copper ketones and hydroxylamines; Cyanide. Benzoic acid and benzyl alcohol are non-competitive inhibitors

Unit definition: at 25 ℃, pH 7.2, one micromole of benzylamine oxide per minute

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.