Dispersion enzymes are metalloproteinases that cut the neutral, non-polar amino acid N end of the peptide chain, capable of stripping epithelial cells from tissue into sheets. Previously, technicians have used it to separate and disperse cells in primary cultures. In recent years, it has also been widely used in the fields of regenerative medicine and tissue engineering, and can be used in combination with collagenase. This enzyme separates cells quickly, efficiently and gently, the cells can remain intact, does not cause damage to the cell membrane, has no animal source raw materials, does not contain mycoplasma or animal virus contamination, and is very stable for serum composition, enzyme concentration, treatment events, temperature, and pH.

Dispersase is a neutral metalloproteinase derived from Paenibacillus sp. (formerly Bacillus polymyxa). The active center of Zn2+ is more stable than Ca2+.

Enzyme activity is almost unaffected by serum composition, so this product can be used with or without serum. At the same time, the enzyme reaction can be stopped simply by adding EDTA or diluting the reaction solution.

It can decompose the type IV collagen and fibronectin that make up the basement membrane well, so that the epithelial cells can be stripped from the tissue into sheets.

Different from trypsin, collagenase and other proteases, it causes less cell damage and can gently disperse cells.

Due to the wide range of conditions of action, enzymes can be activated under different enzyme concentrations, treatment times, temperatures, and pH conditions.

The cell culture temperature is generally 37℃, and the enzyme is very stable at this temperature. Depending on the cell type, this product can be added to the medium for suspension culture.

This product contains dextrin and calcium acetate

This product does not contain mycoplasma.

This product does not use raw materials of animal origin.