Cell Counting KIT-8 (CCK-8) is a rapid, highly sensitive cell activity detection Kit based on WST-8, which is widely used in cell proliferation and cytotoxicity detection. WST-8 is a compound similar to MTT that, in the presence of electron-coupled reagents, can be reduced by some dehydrogenases in the mitochondria to form an orange-yellow water-soluble mazan compound. The more cells proliferate and the faster, the darker the color; The more cytotoxic, the lighter the color. For the same type of cells, the relationship between the color depth and the number of cells is linear within a certain limit.

This kit is a ready-to-use solution without further preparation, and can be directly used in 96-well plate cell experiment without cell washing and collection. The kit can be used for cell proliferation detection induced by cytokines, cytotoxicity detection induced by cytotoxic agents such as anticancer drugs, or some drug-induced cell growth inhibition detection.