SF488 EdU Flow Cytometry Assay Kit (Green Fluorescence)

Cell proliferation detection is a basic experimental tool for evaluating cell health, genotoxicity and the effect of anti-tumor drugs. The most accurate method for detecting cell proliferation is the BrdU method. The EdU test kit is a revolutionary breakthrough of the BrdU method. EdU (5-acetylidene-2 '-deoxyuridine) is a pyrimidine analogue that is integrated into the DNA double strand during DNA synthesis. The EdU method is based on the click reaction, a covalent reaction between a copper-catalyzed azide compound and an alkyne to form a covalent bond.

In this kit, EdU contains alkynes and SF488/555/594/647A Azide dye contains azide compounds. Point-and-click multiplication of EdU tags is fast, efficient and easy to use. The BrdU method requires DNA denaturation (such as acid denaturation, thermal denaturation, or digestion with DNase) to expose BrdU and facilitate BrdU antibody binding. The EdU method only needs to be fixed with paraformaldehyde and promoted by Triton X-100 to make the detection reagent enter the cell, and only a small amount of azide dye can mark the integrated EdU very effectively.

This kit contains all components required for EdU assay and can be used for proliferation detection of cultured cells in vitro.

1. Please centrifuge the product to the bottom of the tube immediately before use, and then conduct subsequent experiments.

2. Fluorescent dyes all have quenching problems. Please avoid light as much as possible during experimental operation to slow down fluorescence quenching.

3.Click-iT EdU buffer additive solution is best prepared on the spot to ensure the best results.

4. For your safety and health, please wear a lab coat and disposable gloves.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.