Dulbecco's Modified Eagle Medium -- DMEM (Dulbecco's Modified Eagle Medium) is developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid is increased by 2 times, the content of vitamin is increased by 4 times. Non-essential amino acids, trace iron ions and sodium pyruvate were also added. DMEM medium was originally designed with a glucose content of 1000mg/L (low glucose type), and later developed with a glucose content of 4500mg/L (high glucose type), and has been widely used in the culture of various cells. DMEM high-glucose medium is widely used in fast-growing, low-adhesion cells, hybridoma myeloma cells, cloned cells, DNA-transfected transformed cells, various primary virus host cells, single cell culture, and vaccine production, such as the use of CHO cells to express EPO and produce hepatitis B vaccine. This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, but does not contain proteins, lipids or any growth factors, so this product should be used with serum or no serum additives.