Endotoxin: ≤1 EU/mL

Osmotic pressure: 270-340 mOsm/kg

pH: 7.2-7.4

Serum addition: 10% fetal bovine serum

Culture condition: 37℃, 5-10% carbon dioxide

Cell type: A variety of suspended and attached mammalian cells

Product Introduction

The carbon dioxide independent medium was buffered by phosphate. The formula contains a small amount of sodium bicarbonate to satisfy the necessary bicarbonate-dependent function. Since synthetic buffers such as Hepes are not used, the cytotoxic effects associated with such buffer systems are eliminated. In addition, the optimized formulation of the medium improves cell production and CO2 utilization, so that CO2-dependent cell function can be maintained without exogenous CO2. CO2 independent media are used to support the growth of a variety of suspended and attached mammalian cell lines, such as epithelial cells, fibroblasts and lymphoid cell lines, without the need for CO2 incubators. The medium is ideal for transporting cells or tissues under atmospheric conditions and for handling mouse embryos. For long-term culture, traditional standard CO2-dependent medium is recommended