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My Cart> Cell Biology > Cell Culture ＆ Digestion > Cell Culture Medium > CO2 Independent Medium, with Phenol Red, L-glutamine, and Sodium Pyruvate, without HEPESStorage：Store at 2-8℃，avoid light，1 year
Appearance：Liquid
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| Appearance | Liquid |
| Storage | Store at 2-8℃，avoid light，1 year |
| Source | Non-animal Origin |
| Unit | Bottle |
| Specification | 500ml |
Endotoxin: ≤1 EU/mL
Osmotic pressure: 270-340 mOsm/kg
pH: 7.2-7.4
Serum addition: 10% fetal bovine serum
Culture condition: 37℃, 5-10% carbon dioxide
Cell type: A variety of suspended and attached mammalian cells
Product Introduction
The carbon dioxide independent medium was buffered by phosphate. The formula contains a small amount of sodium bicarbonate to satisfy the necessary bicarbonate-dependent function. Since synthetic buffers such as Hepes are not used, the cytotoxic effects associated with such buffer systems are eliminated. In addition, the optimized formulation of the medium improves cell production and CO2 utilization, so that CO2-dependent cell function can be maintained without exogenous CO2. CO2 independent media are used to support the growth of a variety of suspended and attached mammalian cell lines, such as epithelial cells, fibroblasts and lymphoid cell lines, without the need for CO2 incubators. The medium is ideal for transporting cells or tissues under atmospheric conditions and for handling mouse embryos. For long-term culture, traditional standard CO2-dependent medium is recommended
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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