Ham'sF-12 Nutrient Mixture was designed by Ham in 1969 based on Ham's F-10 nutrient mixture and was originally used for serum-free culture of CHO cells. Ham's F-12 is often used as a basic culture medium for serum-free culture. It is especially suitable for single cell culture and clonal culture at low serum content. It is also widely used in the culture of cancer cells and primary cells after adding serum, such as rat hepatocytes, rat prostate epithelial cells, chondrocytes, rat myoblasts, and chicken embryonic cells. In addition, after Ham's F-12 is mixed with DMEM in equal volume, the obtained DMEM/F12 medium is more nutritious and more widely used. This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, but does not contain proteins, lipids or any growth factors, so this product should be used with serum or no serum additives.