MEM Medium (Minimum Essential Medium), also known as the minimum essential medium, minimum essential medium or low limit Eagle medium, developed by Harry Eagle on the basis of the Eagle Basic Medium (BEM), is the most basic and most applicable medium. It is one of the most commonly used media for animal cell culture. MEM medium contains only 12 essential amino acids, glutamine and 8 vitamins, the composition is simple, mainly used for the culture of adherent cells, the formula can also be used for other types of cell culture after modification. MEM is a medium containing NEAA (non-essential amino acids), which is based on MEM medium with the addition of L-alanine, L-glutamic acid, L-asparagine, L-aspartate, L-proline, L-serine and glycine 7 kinds of NEAA, which can reduce the side effects of cell production of non-essential amino acids in cell culture. Effectively promote cell proliferation and metabolism. This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, but does not contain proteins, lipids or any growth factors, so this product should be used with serum or no serum additives.