High Glucose-reduced Serum DMEM Medium

Product Introduction

High glucose DMEM added ethanolamine, insulin, transferrin, bovine serum albumin, glutathione, ascorbic acid, and some trace elements on the basis of classic DMEM, which could ensure the cell culture and proliferation under the condition of low serum. This medium is commonly used in attenuated mammalian cell cultures and contains phenol red, 0.11g/L pyruvate and MEN essential amino acids, but does not contain L-amine and HEPES. Compared with classical DMEM, this formula can reduce the amount of serum by more than 50%, and does not affect the cell growth rate and morphology, thus extending the time of use of the same batch of serum, and reduce the fluctuation of culture conditions, but also reduce the infection and transmission of pathogens, thereby improving the purity and reliability of cell culture. The medium can be used on MDBK, A549, MDCK, HepG2, COS-7, Vero and WI-38 cells without acclimation.

Instructions for use

Before use, fetal bovine albumin (1-5%) and 4mM of L-glutamine or alanyl-glutamine should be added. Because different types of cells depend on the serum to different degrees, we recommend optimizing for specific cells to determine the final serum concentration.

Matters needing attention

This product is filtered to remove bacteria, and should pay attention to aseptic operation when using to avoid contamination; In order to maintain the best use of this product, do not freeze and thaw treatment; This product is only for scientific research and production use, for in vitro culture of tissues and cells, clinical use is prohibited; Cells need to be grown in an incubator containing 5 to 10 percent carbon dioxide.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.