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丁酰胆碱酯酶(BchE)活性检测试剂盒

Storage:Store at -20℃,6 months

丁酰胆碱酯酶(BchE)活性检测试剂盒
Cat.No:
BC5970
Brand:
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
BC5970-50T/48S Inquiry Inquiry Inquiry Inquiry
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Butyrylcholinesterase (BchE, EC3.1.1.8), also known as plasma cholinesterase, pseudocholinesterase, is a serine hydrolase that is synthesized by the liver and enters the blood and is present in almost all animal tissues. BchE is structurally similar to acetylcholinesterase (AchE), but with different substrate specificity and inhibitor sensitivity. Compared with AchE, BchE can effectively hydrolyze larger choline esters, such as butyrylcholine and benzoylcholine, and can remove the toxic effect of nerve agents such as organophosphorus pesticides and carbamate pesticides. Studies have shown that BchE can be an important target for the treatment of Alzheimer's disease.

BchE catalyzed the hydrolysis of butyrylcholine to choline, and the reaction of choline with disulfide p-nitrobenzoic acid (DTNB) to 5-merhydryl-nitrobenzoic acid (TNB). TNB has an absorption peak at 412nm, and BchE activity is calculated by measuring the absorbance increase rate at 412nm.

Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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