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丁酰胆碱酯酶抑制剂活性检测试剂盒

BC5985-241212Manual

Cat.No：BC5985 Solarbio

Detection Method：Spectrophotometer/Microplate Reader

Storage：Store at -20℃，6 months

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Product Information

Name	Butyrylcholinesterase Inhibitor Activity Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Unit	Kit
Specification	100T/96S

Butyrylcholinesterase (BchE, EC3.1.1.8), also known as plasma cholinesterase, pseudocholinesterase, is a serine hydrolase that is synthesized by the liver and enters the blood and is present in almost all animal tissues. Compared with acetylcholinesterase (AchE), BchE can effectively hydrolyze larger choline esters, such as butyrylcholine and benzoylcholine, and can remove the toxic effects of nerve agents such as organophosphorus pesticides and carbamate pesticides. Studies have shown that BchE can be used as an important target for the treatment of Alzheimer's disease, and BchE inhibitors are used to improve memory loss and cognitive dysfunction in Alzheimer's patients.

BchE catalyzes the hydrolysis of butyrylcholine to choline, and the reaction of choline with dithio-nitrobenzoic acid (DTNB) to produce 5-merhydryl-nitrobenzoic acid (TNB). BchE inhibitors reduce the hydrolysis of butyrylcholine by inhibiting BchE activity. TNB has an absorption peak at 412nm, and the BchE inhibitor activity can be calculated by measuring the change in absorbance at 412nm.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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