Introduction

Transferrin is the main iron transport protein in plasma, providing iron needed for cell internalization and cell metabolism. Studies have found that all cell growth requires transferrin. Not only does normal cell metabolism depend on iron in its biologically active form, but iron is also a cofactor for enzymes such as RNA polymerase and DNA synthetase. It's also an important component of hemoglobin. Transferrin is a plasma glycoprotein isolated from plasma, bovine transferrin exists in two molecular forms, namely 74KD and 78KD respectively. Each transferrin molecule can bind two molecules of iron ions through the C-terminal and N-terminal structural sites, so transferrin exists in three forms: deferrized (not bound to iron ions), saturated iron (bound to two molecules of iron ions), and partially saturated, under physiological conditions, only 1/3 of transferrin molecules are saturated with iron. This product is derived from bovine plasma, purified by multi-step chromatography, inactivated by 60°C virus, filtered by 0.1 μm filter membrane, and then lyophilized. Through strict quality control (purity, endotoxin, pH, iron content, moisture, microbial contamination, etc.), cell culture requirements are met.

Application

Cell culture; A nutrient additive for fermentation medium of recombinant protein and biopharmaceutical production;

It is used as a molecular weight standard protein or as a receptor to mediate the transfection of molecules such as DNA into cells

How to use

During cell culture, the concentration of transferrin in the medium should be maintained at 0.5-100μg /ml. For different cell lines, the most appropriate concentration needs to be selected. Freeze-dried transferrin powder should be kept in a refrigerated state and dissolved before use. Generally, the freeze-dried transferrin powder is dissolved into a storage solution at room temperature with PBS or other suitable solvent at a concentration of 0.5-2.0mg/ml, and then filtered with a sterile filter membrane.