Liquiritigenin

Examples of using this product（for reference only）

LC-MS

The samples were analyzed by LC-MS system . A 10-ml solution was injected into the LC column (C18 2.6μm 100 ×2.1 mm) with a flow rate of 0.3 ml/min. The column temperature was 4 ℃. Buffer A consisted of 0.1% formic acid in the water, and Buffer B was methanol. The gradient was 5% Buffer B for 1 min,5–95% Buffer B for 14 min, and 95% Buffer B for 3 min.The sample was analyzed by mass spectrometer with directinfusion of the sample into the electrospray ionization source operating in the negative ion mode. Curtain gas, ion source gas and auxiliary gas were 40 psi, 65 psi and 65 psi, respectively.IonSpray voltage floating, collision energy and declustering potential were set to -4500 V, -10 V and -80 V, respectively,with a gasification temperature of 550 ℃.

References：

Song M, Lei Y, Ali A, Xu Y, Sheng K, Huang T, Huang J, Huang M. Inhibitory effect of licorice extract on the germination and outgrowth of Paraclostridium bifermentans spores. Front Microbiol. 2022 Dec 2;13:1076144. doi: 10.3389/fmicb.2022.1076144. PMID: 36532483; PMCID: PMC9755857.

LC-ESI-MS/MS

The chromatographic conditions were as follows: BEH C18 chromatographic column (150?mm?×?2.1?mm, 1.7 μm); mobile phase: A phase water, B phase acetonitrile, 0.1% (vol/vol) formic acid and 0.2 mM ammonium acetate were added t both phases. Step-stripping sequence: 0–1.0 min, 10% B; 0–9.0?min, 10% B–90% B; 9.0–11.0?min, 90% B–100% B; 11.0–11.1?min, 100% B–10% B; 11.1–13.0 min, 10% B. Column temperature: 30℃; injection volume: 2 μl; flow rate: 0.25?ml/min. Mass spectrum conditions: retention time 4.94?min?1; ion pair l: quantitative ion 433.1/271.1, collision energy ?22?V; ion pair 2: quantitative ion 433.1/150.9, collision energy ?42?V.

References：

Sun M, Li L, Wang C, Wang L, Lu D, Shen D, Wang J, Jiang C, Cheng L, Pan X, Yang A, Wang Y, Zhu X, Li B, Li Y, Zhang F. Naringenin confers defence against Phytophthora nicotianae through antimicrobial activity and induction of pathogen resistance in tobacco. Mol Plant Pathol. 2022 Dec;23(12):1737-1750. doi: 10.1111/mpp.13255. Epub 2022 Sep 12. PMID: 36094814; PMCID: PMC9644278.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.