Hoechst 33342 Stain solution(ready-to-use)
Cat.No:C0030 Solarbio
Storage:Store at -20℃,avoid light,1 year
Qty:
Size:
{{cart_num}}
My CartStorage:Store at -20℃,avoid light,1 year
Qty:
Size:
Name | Hoechst 33342 Stain solution(ready-to-use) |
Storage | Store at -20℃,avoid light,1 year |
Unit | Bottle |
Specification | 10ml 50ml |
Hoechst 33342,也称bisBenzimide H 33342或HOE 33342,是一种可以穿透细胞膜的蓝色荧光染料,对细胞的毒性较低。Hoechst 33342染色常用于细胞凋亡检测,染色后用荧光显微镜观察或流式细胞仪检测。Hoechst 33342也常用于普通的细胞核染色,或常规的DNA染色。Hoechst 33342的最大激发波长为346nm,最大发射波长为460nm;Hoechst 33342和双链DNA结合后,最大激发波长为350nm,最大发射波长为461nm。本Hoechst 33342染色液为即用型,可直接用于固定细胞或组织的细胞核染色,也可直接用于活细胞或组织的细胞核染色。
使用说明:
1. 对于固定的细胞或组织:
a. 对于细胞或组织样品,固定后,适当洗涤去除固定剂。随后如果需要进行免疫荧光染色,则先进行免疫荧光染色,染色完毕后再按后续步骤进行Hoechst 33342染色。如果不需要进行其它染色,则直接进行后续的Hoechst 33342染色。
b. 对于贴壁细胞或组织切片,加入少量Hoechst 33342染色液,覆盖住样品即可;对于悬浮细胞,至少加入待染色样品3倍体积的染色液,混匀。室温放置3-5分钟。
c. 吸除Hoechst 33342染色液,用TBST、PBS或生理盐水洗涤2-3次,每次3-5分钟。
d. 直接在荧光显微镜下观察或封片后荧光显微镜下观察。细胞发生凋亡时,会看到凋亡细胞的细胞核呈致密浓染,或呈碎块状致密浓染。
2. 对于活细胞或组织:
a. 加入适当量Hoechst 33342染色液,必须充分覆盖住待染色的样品,通常对于六孔板一个孔需加入1ml染色液,对于96孔板一个孔需加入100微升染色液。
b. 在适宜于细胞培养的温度下培养20-30分钟。弃染色液,用PBS或培养液洗涤2-3次即可进行荧光检测。
注意事项:
荧光染料都存在淬灭的问题,为减缓荧光淬灭可以使用抗荧光衰减封片剂。建议染色后尽量当天完成检测,活细胞或组织染色后应立即观察。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no experimental images.
Sorry, there is no more information.
Rapid Surface Display of mRNA Antigens by BacteriaDerived Outer Membrane Vesicles for a Personalized Tumor Vaccine
Click to check >>Author:李瑶
IF:30.8000
Publish_to:Advanced Materials
PMID:35315546
A PDA-Functionalized 3D Lung Scaffold Bioplatform to Construct Complicated Breast Tumor Microenvironment for Anticancer Drug Screening and Immunotherapy
Click to check >>Author:Wanheng Zhang, Yan Chen, Mengyuan Li, Shucheng Cao, Nana Wang, Yingjian Zhang, Yongtao Wang
IF:15.1000
Publish_to:Advanced Science
PMID:37424037
Bacterial Outer Membrane Vesicles Presenting Programmed Death 1 for Improved Cancer Immunotherapy via Immune Activation and Checkpoint Inhibition
Click to check >>Author:李瑶
IF:14.5880
Publish_to:
PMID:33232124
Engineered Platelet-Based Micro/Nanomotors for Cancer Therapy
Click to check >>Author:李婷
IF:13.2810
Publish_to:Small
PMID:34741421
Antigen Capture and Immune Modulation by Bacterial Outer Membrane Vesicles as In Situ Vaccine for Cancer Immunotherapy Post-Photothermal Therapy
Click to check >>Author:李瑶(Yao Li)
IF:13.2000
Publish_to:small
PMID:35152555
Bioengineered bacteria-derived outer membrane vesicles as a versatile antigen display platform for tumor vaccination via Plug-and-Display technology
Click to check >>Author:程科满
IF:12.1210
Publish_to:
PMID:33824314
Autophagy-induced intracellular signaling fractional nano-drug system for synergistic anti-tumor therapy
Click to check >>Author:Jianqin Yan, Chan Shan, Zhuoran Zhang, Fashun Li, Yong Sun, Qian Wang, Bin He, Kui Luo, Jing Chang, Yan Liang
IF:9.9650
Publish_to:JOURNAL OF COLLOID AND INTERFACE SCIENCE
PMID:37179196
Consuming intracellular glucose and regulating the levels of O2/H2O2 via the closed cascade catalysis system of Cu-CeO2 nanozyme and glucose oxidase
Click to check >>Author:Jialing Gui, Haoyu Chen, Jing Liu, Yani Liu, Cuiyan Wu, Xiaohua Zhu, Mingjie Wei, Meiling Liu, Youyu Zhang, Shouzhuo Yao
IF:9.9000
Publish_to:JOURNAL OF COLLOID AND INTERFACE SCIENCE
PMID:37542894
Framework Nucleic Acid-Based Multifunctional Tumor Theranostic Nanosystem for miRNA Fluorescence Imaging and Chemo/Gene Therapy
Click to check >>Author:Haikun Luo, Zhao Wang, Qian Mo, Jianying Yang, Fan Yang, Yujin Tang, Jia Liu, Xinchun Li
IF:9.5000
Publish_to:ACS Applied Materials & Interfaces
PMID:37421332
NPLC0393 from Gynostemma pentaphyllum ameliorates Alzheimers disease-like pathology in mice by targeting protein phosphatase magnesium-dependent 1A phosphatase
Click to check >>Author:Jianlu Lv, Xingyi Shen, Xinya Shen, Shimei Zhao, Rui Xu, Qiuying Yan, Jian Lu, Danyang Zhu, Yonghua Zhao, Jiajia Dong, Jiaying Wang, Xu Shen
IF:7.2000
Publish_to:PHYTOTHERAPY RESEARCH
PMID:37434441
Characterization and screening of anti-melanogenesis and anti-photoaging activity of different enzyme-assisted polysaccharide extracts from Portulaca oleracea L.
Click to check >>Author:Xingyu Tao, Xuan Hu, Tongchuan Wu, Dongyue Zhou, Di Yang, Xue Li, Yunhua Fu, Fei Zheng, Hao Yue, Yulin Dai
IF:6.6560
Publish_to:PHYTOMEDICINE
PMID:37229889
Adiponectin reduces ER stress-induced apoptosis through PPARα transcriptional regulation of ATF2 in mouse adipose
Click to check >>Author:Zhenjiang Liu1,3, Lu Gan1,3, Tianjiao Wu1, Fei Fen
IF:5.9650
Publish_to:Cell Death and Disease (2016) 7, e2487
PMID:27882945
The potential role of the HCN1 ion channel and BDNF-mTOR signaling pathways and synaptic transmission in the alleviation of PTSD
Click to check >>Author:倪良卉
IF:5.6810
Publish_to:
PMID:32198387
Microfluidic Formulation of Curcumin-Loaded Multiresponsive Gelatin Nanoparticles for Anticancer Therapy
Click to check >>Author:Yu Xia, Ruicheng Xu, Siyuan Ye, Jiaxuan Yan, Piyush Kumar, Peng Zhang, Xiubo Zhao
IF:5.3950
Publish_to:ACS Biomaterials Science & Engineering
PMID:37140447
Staphylococcus aureus extracellular vesicles induce apoptosis and restrain mitophagy-mediated degradation of damaged mitochondria
Click to check >>Author:Xiaozhou Wang, Hongyan Li, Jie Wang, Huiling Xu, Kun Xue, Xiaoting Liu, Zhizhong Zhang, Jianzhu Liu, Yongxia Liu
IF:5.0700
Publish_to:MICROBIOLOGICAL RESEARCH
PMID:37267814
Macrocyclic polyamine [12]aneN3 modified triphenylamine-pyrazine derivatives as efficient non-viral gene vectors with AIE and two-photon imaging properties
Click to check >>Author:马乐乐
IF:5.0470
Publish_to:
PMID:32222754
Matrix stiffness induces an invasive-dormant subpopulation via cGAS-STING axis in oral cancer
Click to check >>Author:Li Jingyuan, Liu Yu, Jiang Hong, Wang Tao, Li Kan, Lao Xiaomei, Liao Guiqing, Liang Yujie
IF:4.8030
Publish_to:Translational Oncology
PMID:37137218
Chemopreventive Role of Apigenin against the Synergistic Carcinogenesis of Human Papillomavirus and 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone
Click to check >>Author:尹方正
IF:4.7100
Publish_to:
PMID:33158065
Electrical stimulation induced structural 3D human engineered neural tissue with well-developed neuronal network and functional connectivity
Click to check >>Author:Xiaoting Meng, Xiyao Yu, Yingli Lu, Zhe Pei, Guoqiang Wang, Mingran Qi, Rongrong Liu, Jiaying Zhou, Xiaopin Guo, Zhengjie Zhou, Fang Wang
IF:4.0000
Publish_to:Journal of Neural Engineering
PMID:37433290
ameliorative effect of recombinant human lactoferrin on the premature ovarian failure in rats after cyclophosphamide treatments
Click to check >>Author:Gang LIU
IF:3.5000
Publish_to:Journal of Ovarian Research
PMID:33478578
Quantitative analysis of the effects of vimentin intermediate filaments on the early stages of influenza A virus infection
Click to check >>Author:Ju-Mei Zhang, Zhi-Gang Wang, Lei Du, Dan-Dan Fu, Meng-Qian Zhang, Jing Li, Shu-Lin Liu, Dai-Wen Pang
IF:3.3000
Publish_to:NEW JOURNAL OF CHEMISTRY
PMID:
ZnSO4 Protects against premature ovarian failure through PI3K/AKT/GSK3β signaling pathway
Click to check >>Author:Zhe Dong, Lu Zhang, Wei Wang, Fan Jiang, Hao Ai
IF:2.9230
Publish_to:THERIOGENOLOGY
PMID:37269597
Protective Effect of Cannabidiol on Hydrogen Peroxide-Induced Oxidative Damage in Human Umbilical Vein Endothelial Cells (HUVECs)
Click to check >>Author:Hang Li, Tian-Xiao Yang, Qing-Sheng Zhao, Bing Zhao
IF:2.9000
Publish_to:CHEMISTRY & BIODIVERSITY
PMID:37382028
Downregulation of circular RNA hsa_circ_0087856 sensitizes bladder cancer cells to cisplatin through targeting miR-1184/CITED2 signaling
Click to check >>Author:Min Ju, Weiwei Wu, Jingkun Qu, Yang Sun, Jun Li
IF:2.8000
Publish_to:ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
PMID:37392146