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Your Position: Home > Standard References > Standard References > L-Tryptophan
L-Tryptophan

CAS:73-22-3

Appearance:White to off-white powder

Storage:RT.

Purity:HPLC≥98%

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L-Tryptophan
Cat.No：
ST8320
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
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Product Information Related Products Related Paper COA Technical Q&A
Examples of using this product（for reference only）

HPLC-MS

HPLC was performed using an HPLC-MS instrumen for detection and quantitation of tryptophan and kynurenine. The mass scan mode was set to positive and negative ions, and the range was set to 70–1000 m/z. Separation of tryptophan and kynurenine was carried out on an C18 column. Standards and samples were eluted using methyl alcohol and ultrapure water (0.1% formic acid). The injection volume was set to 5 μL, with a solvent flow rate of 0.2 mL/min. The column temperature was set to 40 ℃. The IDA mode was used for data acquisition.

References：

Ai Y, Wang B, Xiao S, Luo S, Wang Y. Tryptophan Side-Chain Oxidase Enzyme Suppresses Hepatocellular Carcinoma Growth through Degradation of Tryptophan. Int J Mol Sci. 2021 Nov 18;22(22):12428. doi: 10.3390/ijms222212428. PMID: 34830310; PMCID: PMC8623686.

HPLC

The SA content was determined using high-performance liquid chromatography with a 4.6 × 250 mm column . The mobile phase had a flow rate of 0.7 mL/min (isocratic elution) and was composed of acetonitrile and 0.1% phosphoric acid (97:3, v/v). The column temperature was 25 ℃, a UV–visible detector was used for detection at a wavelength of 213 nm, and the injection volume was 20 μL.

References：

Shi Y, Chen G, Chen K, Chen X, Hong Q, Kan J. Assessment of fresh star anise (Illicium verum Hook.f.) drying methods for influencing drying characteristics, color, flavor, volatile oil and shikimic acid. Food Chem. 2021 Apr 16;342:128359. doi: 10.1016/j.foodchem.2020.128359. Epub 2020 Oct 13. PMID: 33092919.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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