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Application：ELISA
Reactivity：Rat
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：15.625 pg/ml
Storage：2-8℃，6 months
Gene ID：84398
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| Name | Rat CD93 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | CD93 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Rat |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 31.25-2000 pg/ml |
| Sensitivity | 15.625 pg/ml |
| Specificity | Do not react with Rat CD93 analogue etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | Q9ET61 |
| Gene ID | 84398 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠CD93单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠CD93会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗大鼠CD93抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠CD93发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠CD93，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠CD93浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠CD93的浓度。 |
| Background | CD93 or C1q receptor 1 (C1qR) is an about 120 kDa O-sialoglycoprotein that within the hematopoietic system is selectively expressed on cells of the myeloid lineage. CD93/C1qR is a highly glycosylated transmembrane protein expressed on monocytes，neutrophils，endothelial cells，and stem cells. CD93 was originally identified as a myeloid cell-surface marker and subsequently associated with an ability to modulate phagocytosis of suboptimally opsonized immunoglobulin G and complement particles in vitro. CD93/C1qR，a receptor expressed during early B-cell development，is reinduced during plasma-cell differentiation. High CD93/CD138 expression was restricted to antibody-secreting cells both in T-dependent and T-independent responses as naive，memory，and germinal-center B cells remained CD93-negative. CD93 was expressed on (pre)plasmablasts/plasma cells，including long-lived plasma cells that showed decreased cell cycle activity，high levels of isotype-switched Ig secretion，and modification of the transcriptional network. CD93 is important for the maintenance of plasma cells in bone marrow niches |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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