{{cart_num}}
My Cart
Application：ELISA
Reactivity：Rat
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：9 pg/ml
Storage：2-8℃，6 months
Gene ID：25544
Qty：
Size：
| Name | Rat E-Selectin ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | E-Selectin |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Rat |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 18.75-1500 pg/ml |
| Sensitivity | 9 pg/ml |
| Specificity | Do not react with Rat E-Selectin analogue etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | P98105 |
| Gene ID | 25544 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠E-Selectin单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠E-Selectin会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗大鼠E-Selectin抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠E-Selectin发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠E-Selectin，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠E-Selectin浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠E-Selectin的浓度。 |
| Background | E-selectin，also known as endothelial leukocyte adhesion molecule-1 (ELAM-1)and CD62E，is an inducible adhesion molecule that is expressed on the surfaces of stimulated vascular endothelial cells and is sometimes involved in cancer cell metastasis. E-selectin exhibits a complex mosaic structure consisting of a large extracellular region comprised of a lectin domain，an EGF-like domain，and a short consensus repeat (SCR) domain，followed by a transmembrane region and a relatively short (32 aa) cytoplasmic tail. As a member of the LEC-CAM or selectin family，E-selectin recognises and binds to sialylated carbohydrates including members of the Lewis X and Lewis A families found on monocytes，granulocytes，and T-lymphocytes. E-selectin supports rolling and stable arrest of leukocytes on activated vascular endothelium，and furthermore，it was indicated that it can also transduce an activating stimulus via the MAPK cascade into the endothelial cell during leukocyte adhesion. E-selectin regulates adhesive interactions between certain blood cells and endothelium. The soluble form of E selectin (sE-selectin) is a marker of endothelial activation，and has a potential role in the pathogenesis of cardiovascular disease as raised levels have been found in hypertension，diabetes and hyperlipidemia，although its association in established atherosclerosis disease and its value as a prognostic factor is more controversial. soluble E-selectin is inversely associated with the muscular component of the left ventricle，thereby suggesting that the lack of such a reparative factor may be associated with cardiac remodeling in end-stage renal disease (ESRD) patients. In addition，this adhesion molecule appears to be involved in the pathogenesis of atherosclerosis. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no experimental images.
Sorry, there is no more information.
Sorry, there is no more information.
