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Application：ELISA
Reactivity：Rat
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：12 pg/ml
Storage：2-8℃，6 months
Gene ID：25114
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| Name | Rat FGFR4 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | FGFR4 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Rat |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 23.43-1500 pg/ml |
| Sensitivity | 12 pg/ml |
| Specificity | Do not react with Rat FGFR4 analogue etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | Q498D6 |
| Gene ID | 25114 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠 FGFR4单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠 FGFR4会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗大鼠 FGFR4抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠 FGFR4发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠 FGFR4，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠 FGFR4浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠 FGFR4的浓度。 |
| Background | Fibroblast growth factor receptor 4 (FGFR4) also known as CD334 antigen or tyrosine kinase related to fibroblast growth factor receptor，is a member of the fibroblast growth factor receptor family，where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region，composed of three immunoglobulin-like domains，a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of FGFR4/CD334 interacts with fibroblast growth factors，setting in motion a cascade of downstream signals，ultimately influencing mitogenesis and differentiation. FGFR4/CD334 preferentially binds acidic fibroblast growth factor and，although its specific function is unknown，it is overexpressed in gynecological tumor samples，suggesting a role in breast and ovarian tumorigenesis. FGFR4/CD334 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4/CD334. Mutations in FGFR4/CD334 lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
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