{{cart_num}}
My Cart
Application：ELISA
Reactivity：Human
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：15.625 pg/ml
Storage：2-8℃，6 months
Gene ID：2219
Qty：
Size：
| Name | Human FCN1 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | ficolin 1/FCN1 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Human |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 31.25-2000 pg/ml |
| Sensitivity | 15.625 pg/ml |
| Specificity | Do not react with Human FCN1 analogue etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | O00602 |
| Gene ID | 2219 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人FCN1单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人FCN1会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗人FCN1抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人FCN1发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人FCN1，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人FCN1浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人FCN1的浓度。 |
| Background | Ficolins are humoral molecules of the innate immune systems which recognize carbohydrate molecules on pathogens，apoptotic and necrotic cells. The Ficolin family of proteins are characterized by the presence of a leader peptide，a short N-terminal segment，followed by a collagen-like region，and a C-terminal fibrinogen-like domain. Ficolins are humoral molecules of the innate immune systems which recognize carbohydrate molecules on pathogens，apoptotic and necrotic cells. Three Ficolins have been identified in humans: L-Ficolin，H-Ficolin and M-Ficolin (also referred to as Ficolin-2，-3 and -1，respectively). They are soluble oligomeric defence proteins with lectin-like activity and they are structurally similar to the human collectins，mannan-binding lectin (MBL) and surfactant protein A and D. Dysfunction or abnormal expressions of Ficolins may involved in the pathogenesis of human diseases including infectious and inflammatory diseases，autoimmune disease and clinical syndrome of preeclampsia. They are soluble oligomeric defence proteins with lectin-like activity and they are structurally similar to the human collectins，mannan-binding lectin (MBL) and surfactant protein A and D. Upon recognition of the infectious agent，the Ficolins act through two distinct routes: initiate the lectin pathway of complement activation through attached serine proteases (MASPs)，and a primitive opsonophagocytosis thus limiting the infection and concurrently orchestrating the subsequent adaptive clonal immune response. Ficolin-1 (FCN1) is predominantly expressed in the peripheral blood leukocytes. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no experimental images.
Sorry, there is no more information.
Sorry, there is no more information.
