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Application：ELISA
Reactivity：Rat
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：6.25 pg/ml
Storage：2-8℃，6 months
Gene ID：117022
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| Name | Rat IL1R2 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | IL1R2 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Rat |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 12.5-800 pg/ml |
| Sensitivity | 6.25 pg/ml |
| Specificity | Do not react with Rat IL-1R2 analogue etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | P43303 |
| Gene ID | 117022 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠 IL-1R2单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠IL-1R会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗大鼠 IL-1R2抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠 IL-1R2发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠 IL-1R2，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠 IL-1R2浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠 IL-1R2的浓度。 |
| Background | Interleukin 1 receptor，type II (IL1R2) also known as CD121b (Cluster of Differentiation 121b) is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein binds interleukin alpha (IL1A)，interleukin beta (IL1B)，and interleukin 1 receptor，type I (IL1R1/IL1RA)，and acts as a decoy receptor that inhibits the activity of its ligands. The pleiotropic cytokine IL1 is produced to regulate the development and maintenance of the inflammatory responses and binds to specific plasma membrane receptors on cells. Two distinct types of IL1 receptors that can bind IL1 specifically have been identified，designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R1 contributes to IL-1 signaling，whereas the IL-1R2/CD121b has no signaling property and acts as a decoy for IL-1. IL-1R2/CD121b structurally consisting of a ligand-binding portion comprised of three Ig-like domains，a single transmembrane region，and a short cytoplasmic domain is expressed in a variety of cell types including B lymphocytes，neutrophils，monocytes，large granular leukocytes，and endothelial cells. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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