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Application：ELISA
Reactivity：Human
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：7.8 pg/ml
Storage：2-8℃，6 months
Gene ID：10871
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| Name | Human CD300C ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | CD300C |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Human |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 15.625-1000 pg/ml |
| Sensitivity | 7.8 pg/ml |
| Specificity | Do not react with Human CD300C analogue. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | Q08708 |
| Gene ID | 10871 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人CD300C单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人CD300C会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗人CD300C抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人CD300C发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人CD300C，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人CD300C浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人CD300C的浓度。 |
| Background | The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD300 is a glycoprotein family of cell surface molecules that regulate a diverse array of cellular processes via their triggering and inhibitory receptor functions. The CD300 family of myeloid immunoglobulin receptors includes activating(CD300b，CD300e) and inhibitory members(CD300a，CD300f)，as well as CD300c and CD300d，whose function is uncertain. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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