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Application：ELISA
Reactivity：Human
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：14 pg/ml
Storage：2-8℃，6 months
Gene ID：340205
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| Name | Human TREML1 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | TLT-1/TREML1 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Human |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 28.12-1800 pg/ml |
| Sensitivity | 14 pg/ml |
| Specificity | Do not react with Human TLT-1 analogue etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | Q86YW5 |
| Gene ID | 340205 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人TLT-1单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人TLT-1会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗人TLT-1抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人TLT-1发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人TLT-1，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人TLT-1浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人TLT-1的浓度。 |
| Background | Trem-like transcript 1 protein，also known as Triggering receptor expressed on myeloid cells-like protein 1，TREML1 and TLT-1，is a cytoplasm and single-pass type I membrane protein. TREML1 / TLT-1 is expressed exclusively in platelets and megakaryocytes (MKs) and that its expression is up-regulated dramatically upon platelet activation. It is a receptor that may play a role in the innate and adaptive immune response. TREML1 / TLT-1 contains the characteristic single V-set immunoglobulin (Ig) domain，its longer cytoplasmic tail is composed of both a proline-rich region and an immune receptor tyrosine-based inhibitory motif，the latter known to be used for interactions with protein tyrosine phosphatases. The triggering receptors expressed on myeloid cells (TREMs) have drawn considerable attention due to their ability to activate multiple cell types within the innate immune system，including neutrophils，monocyte / macrophages，and dendritic cells，via their association with DAP12. TREML1 / TLT-1 is prepackaged，along with CD62P，into both MK and platelet alpha-granules. Differences in thrombin-induced redistribution of CD62P and TREML1 indicate that TREML1 is not simply cargo of alpha-granules but may instead regulate granule construction or dispersal. TREML1 / TLT-1 does not function to inhibit members of the TREM family but instead may play a role in maintaining vascular hemostasis and regulating coagulation and inflammation at sites of injury. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
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