EHA105 Electrocompetent cell
Cat.No：C3650 Solarbio
Storage：Store at -70℃
Appearance：Dry ice transport, only 10kg dry ice fee
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> Molecular Biology > Recombinant Clone Expression > Competent Cell > EHA105 Electrocompetent cell
Product Information
| Name | EHA105 Electrocompetent cell |
| Appearance | Dry ice transport, only 10kg dry ice fee |
| Storage | Store at -70℃ |
| Competence | C58 (RifR) Ti pEHA105 (pTiBo542DT-DNA) (StrR)，Succinamopine type |
| Product Description | EHA105 was modified from EHA101 strain (Hood et al., 1993), which was the background of Agrobacterium C58 and contained favorable fampicin resistance gene (Rif) in its nuclear gene. The strain also carries a disarmed Ti plasmid, which has lost its self-transport function. Strain EHA105 contains the carmine type Ti plasmid pEHA105(pTiBo542DT-DNA), which contains the vir gene (vir gene is the necessary element for TDNA insertion into the plant genome), and the T-DNA transfer function of the pEHA105(pTiBo542DT-DNA) plasmid is damaged. However, it can assist the transfer of T-DNA of plant binary expression vector). The pEHA105 (pTiBo542DT-DNA) plasmid also contained Str resistance genes, which conferred streptomycin resistance to EHA105 strains. Agrobacterium EHA105 is suitable for transgenic operation of rice, tobacco and other plants. EHA105 is especially suitable for the transformation of large plasmids, and the transformation efficiency measured by pCAMBIA2301 plasmid is greater than 105CFU/μg DNA. |
| Note | 1. When mixing the plasmid，it should be done gently，and the volume of the plasmid added should not be greater than 1/10 of the volume of the receptive state. The conversion efficiency will be sharply reduced if the plasmid is not pure or very large. 2. When there are too many colonies on the plate，the colonies are small. In order to obtain a large colony，the amount of plasmid used should be reduced or the amount of coating should be reduced，or the colony should be transferred to a new plate for growth. 3. The working concentration of rifampicin should not be higher than 25 μg/ml，too high rifampicin concentration will reduce the growth rate and conversion efficiency. 4. Rifampin has the effect of preventing the growth of miscellaneous bacteria and screening Agrobacterium;Adding streptomycin or gentamicin according to the resistance of the strain used can prevent the loss of Ti plasmid，but streptomycin is not conducive to the transgenic operation of Agrobacterium，so the addition of streptomycin or gentamicin can not be considered in the general culture of agrobacterium，and the probability of Ti plasmid loss is very low (negligible). |
| Unit | Bag |
| Specification | 20*50ul |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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