OrigamiB(DE3)pLysS Competment Cell
Cat.No：C3720 Solarbio
Storage：Store at -70℃
Appearance：Dry ice transport, only 10kg dry ice fee
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Oscillating Mixing & Stirring & Dispersing Instrument
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> Molecular Biology > Recombinant Clone Expression > Competent Cell > OrigamiB(DE3)pLysS Competment Cell
Product Information
| Name | OrigamiB(DE3)pLysS Competment Cell |
| Appearance | Dry ice transport, only 10kg dry ice fee |
| Storage | Store at -70℃ |
| Competence | F-ompT hsdSB(rB-mB-) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB (KanR，TetR) pLysS CamR |
| Product Description | The Origami B (DE3) pLysS strain carries pLysS plasmid and is resistant to chloramphenicol. pLysS can express T7 lysozyme (T7 lysozyme can act on peptidoglycan on Escherichia coli cell wall to dissolve Escherichia coli, and can bind to T7 RNA polymerase to inhibit its transcriptional activity, thereby reducing the background expression level of target genes, but does not interfere with IPTG induced expression), and is suitable for the expression of toxic and non-toxic proteins. Abrupt changes in thioredoxin reductase (trxB) and glutathione reductase in OrigamiB (DE3) pLysS strains (gor), these two enzymes are key enzymes in the reduction pathway, which are conducive to the formation of correctly folded proteins containing disulfide bonds after mutation, and enhance the solubility of the protein. In addition, the chromosome of this strain integrates the λ phage DE3 region (DE3 region contains T7 phage RNA polymerase), which can simultaneously express T7 RNA polymerase and Escherichia coli RNA polymerase, and can be used for protein expression of pET series, pGEX, pMAL and other plasmids. It is resistant to chloramphenicol, kanamycin and tetracycline, and cannot be used for the expression of anti-plasmid with chloramphenicol and kanamycin. OrigamiB (DE3) pLysS receptive cells were made by a special process, and the conversion efficiency of pUC19 plasmid was greater than 107CFU/μg DNA |
| Note | 1，the receptive cells should be stored at -70℃，not repeatedly frozen and thawed and placed for too long，in order to avoid reducing the conversion efficiency of the receptive cells. 2，the conversion operation should be carried out according to the requirements of the corresponding temperature and aseptic conditions. 3. In order to prevent the conversion experiment from being successful，part of the connected reaction liquid can be retained to re-convert and minimize the loss. |
| Unit | Bag |
| Specification | 20*100ul |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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