JM108 Competent cells
Cat.No：C3750 Solarbio
Storage：Store at -70℃
Appearance：Dry ice transport, only 10kg dry ice fee
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PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
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DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
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> Molecular Biology > Recombinant Clone Expression > Competent Cell > JM108 Competent cells
Product Information
| Name | JM108 Competent cells |
| Appearance | Dry ice transport, only 10kg dry ice fee |
| Storage | Store at -70℃ |
| Prokaryotic Resistance | Sensitive to Amp，Kan，Spe，Chl and Tet. Resistant to Nalidixic acid. |
| Competence | F-endA1recA1gyrA96thi-1relA1glnV44Δ(lac-proAB)hsdR17(rK-mK+) |
| Product Description | JM108 is a strain of E. coli K12. Genetic marker gyrA96 indicated that DNA rotation enzyme (topoisomerase II) was mutated, and the mutated topoisomerase II promoted the conformational superhelix of plasmid DNA, and the proportion of superhelix plasmids in cells was greatly increased. endA1 deletion increased the yield and quality of plasmid DNA. Recombinant enzyme defect type (recA1) reduces the probability of homologous recombination of inserted fragments. The insertion of IS186 transposons leads to the deficiency of lon protease expression. JM108 strain is very suitable for construction of recombinant plasmid and extraction of high quality superhelical conformation plasmid. JM108 receptor cells were made by a special process, and the conversion efficiency of pUC19 plasmid was 1×108CFU/μgDNA. |
| Note | 1，the receptive cells should be stored at -70℃，not repeatedly frozen and thawed and placed for too long，in order to avoid reducing the conversion efficiency of the receptive cells. 2，the conversion operation should be carried out according to the requirements of the corresponding temperature and aseptic conditions. 3. In order to prevent the conversion experiment from being successful，part of the connected reaction liquid can be retained to re-convert and minimize the loss. |
| Unit | Bag |
| Specification | 20*100ul |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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