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My CartReactivity：Human,Mouse
Application：IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：79132
Host：Rabbit
Qty：
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| Name | Anti-DHX58 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | IHC 1:50-200. |
| Target | DHX58 |
| Background | Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1-dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells，or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA，binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1，competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3. Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses and coronavirus SARS-CoV-2，and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA，with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA，although it can recognize RNA lacking a 5'-triphosphate. |
| Swiss Prot | Q96C10 |
| Gene ID | 79132 |
| Subcellular Location | Cytoplasm |
| Immunogen | A synthetic peptide of human DHX58 |
| Public Immunogen Range | 330-380/678aa |
| Calculated MW | 77kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse |
| Application | IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with DHX58 antibody diluted at 1:100.
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