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My CartReactivity：Human,Rat,Mouse
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：8065
Host：Rabbit
Qty：
Size：
| Name | Anti-CUL5 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Rabbit |
| Dilution Ratio | WB 1:1000-3000. IHC 1:50-200. |
| Target | CUL5 |
| Background | Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein E3 ubiquitin-protein ligase complexes，which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1 seems to direct ubiquitination of JAK2. ECS(KLHDC1 complex is part of the DesCEND (destruction via C-end degrons pathway and mediates ubiquitination and degradation of truncated SELENOS selenoprotein produced by failed UGA/Sec decoding，which ends with a glycine. May form a cell surface vasopressin receptor. |
| Swiss Prot | Q93034 |
| Gene ID | 8065 |
| Subcellular Location | Nucleus Cytoplasm |
| Immunogen | Recombinant protein of human CUL5 |
| Public Immunogen Range | 1-350aa |
| Calculated MW | 91kDa |
| Observed MW | 91kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with CUL5 antibody diluted at 1:2000;Lane: Jurkat,SH-SY5Y.
Immunohistochemistry of paraffin-embedded Human testis cancer with CUL5 antibody diluted at 1:100.
Immunohistochemistry of paraffin-embedded Human endometrial cancer with CUL5 antibody diluted at 1:100.
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