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My Cart> Cytometric Bead Array > Human CBA Kit > Human Cytokine FlowCytometric Bead Array(CBA) Kit
Detection Method：See the instructions for details
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| Name | Human Cytokine FlowCytometric Bead Array(CBA) Kit |
| Optional Factor Category | TNF-α/IFN-γ/IL-1β/IL-2/IL-4/IL-6/IL-8/IL-10/IL-12p70/IL-17 |
| Detection Sample | Human Serum, Plasma, Culture Supernatant |
| Adaptive Model | This product is suitable for most flow cytometers with PE and APC channels on the market. |
| Sample Requirement | Serum, plasma, cell culture supernatant: It is recommended to test within 4-6 hours, if it cannot be tested in time, please freeze at -25℃, avoid repeated freezing and thawing. |
| Detection Method | See the instructions for details |
| Reference Interval | See the instructions for details |
| Unit | Kit |
| Optional Factor Number | Single Factor |
| Sample Species | Human |
| Detection Principle | This kit uses CBA multifactor flow detection technology, and the fluorescence codes of microspheres are different. Package is respectively on different microspheres with TNF alpha/IFN - gamma/IL - 1 beta/IL - 2 / IL - 4 / IL - 6, IL - 8 / IL - 10 per IL - 12 p70 / IL - 17 specific antibodies, and incubated with the sample under test, with the sample of TNF alpha/IFN - gamma/IL - 1 beta/IL - 2 / IL - 4 / IL - 6 /IL-8/IL-10/IL-12p70/IL-17 specific binding, followed by the addition of biotin-labeled detection antibody, antibody coated microsphere-cytokine - detection antibody immune complex; Finally, phycoregiin-labeled streptavidin was added and combined with biotin. The fluorescence intensity of the tested substance was obtained by flow cytometry. The fluorescence intensity was proportional to the content of TNF-α/IFN-γ/IL-1β/IL-2/IL-4/IL-6/IL-8/IL-10/IL-12p70/IL-17 in the sample. Finally, the standard curves of these ten cytokine standards were combined to achieve the quantitative detection of TNF-α /IFN-γ/IL-1β/IL-2/IL-4/IL-6/IL-8/IL-10/IL-12p70/IL-17 in the same sample. So as to help judge the immune function state of the body. |
| Component 1 | Mixture of Trapping Microsphere |
| Component 2 | Mixture of Detect Antibody |
| Component 3 | Standard |
| Component 4 | PE-labeled Streptavidin |
| Component 5 | Blank Microsphere |
| Component 6 | Standard/Sample Diluent |
| Component 7 | 20× Washing Solution |
| Component 8 | Microporous Plate |
| Component 9 | Sealing Film |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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