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My Cart> Cytometric Bead Array > Mouse CBA Kit > Mouse Cytokine FlowCytometric Bead Array(CBA) Kit
Detection Method：See the instructions for details
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| Name | Mouse Cytokine FlowCytometric Bead Array(CBA) Kit |
| Optional Factor Category | TNF-α/IFN-γ/IL-1b/IL-4/IL-5/IL-6/IL-10/IL-12/IL-17A/GM-CSF/MCP-1 |
| Detection Sample | Mouse Derived Serum, Plasma And Cell Culture Supernatant |
| Adaptive Model | This product is suitable for most flow cytometers with PE and APC channels on the market. |
| Sample Requirement | Serum, plasma, cell culture supernatant: It is recommended to test within 4-6 hours, if it cannot be tested in time, please freeze at -31℃, avoid repeated freezing and thawing. |
| Detection Method | See the instructions for details |
| Reference Interval | See the instructions for details |
| Unit | Kit |
| Optional Factor Number | Single Factor |
| Sample Species | Mouse |
| Detection Principle | This kit uses CBA multifactor flow detection technology, different fluorescence codes of microspheres, different microspheres coated with mouse TNF-α/IFN-γ/IL-4/IL-5/IL-6/IL-10/IL-12/MCP-1/GM-CSF/IL-17A/IL-1β specific antibodies, incubated with the samples to be tested. It can bind specifically to mouseTNF-α/IFN-γ/IL-4/IL-5/IL-6/IL-10/IL-12/MCP-1/GM-CSF/IL-17A/IL-1β in the sample, and then add biotin-labeled detection antibodies to form an antibody coated microglobe-cytokine-detection antibody immune complex. Finally, phycoglobin labeled streptavidin was added and combined with biotin. The fluorescence intensity of the tested substance was obtained by flow cytometry. The fluorescence intensity was directly proportional to the content of mouse TNF-α/IFN-γ/IL-4/IL-5/IL-6/IL-10/IL-12/MCP-1/GM-CSF/IL-17A/IL-1β in the sample, and finally combined with the standard curve of these 11 cytokines standards, Thus, the quantitative detection of mouse TNF-α/IFN-γ/IL-4/IL-5/IL-6/IL-10/IL-12/MCP-1/GM-CSF/IL-17A/IL-1β in the same sample was realized. So as to help judge the immune function state of the body. |
| Component 1 | Mixture of Trapping Microsphere |
| Component 2 | Mixture of Detect Antibody |
| Component 3 | Standard |
| Component 4 | PE-labeled Streptavidin |
| Component 5 | Blank Microsphere |
| Component 6 | Standard/Sample Diluent |
| Component 7 | 20× Washing Solution |
| Component 8 | Microporous Plate |
| Component 9 | Sealing Film |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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