Melatonin

Examples of using this product（for reference only）

LC-MS

For HILIC separation, samples were injected onto an column (2.1 ×100 mm, 1.7 μm; ) at 25 ℃, and using a 21.2 min linear gradient at 0.3 mL/min flow rate and 2 μL injection volume. ESI source conditions were set as follows: curtain gas (CUR) as 30, ion source gas1 (GS1) as 60, and ion source gas2 (GS2); source gas temperature as 600℃; ISVF as 5500 V. The MS/MS acquisition was performed in positive (PI) and negative ion (NI) modes, the mass range was set at 25-1000 m/z, and the accumulation time for product ion scan was set at 0.05 s/spectrum.The second order spectrum was acquired in information-dependent acquisition (IDA) and high-sensitivity modes. Parameters were set as follows: declustering potential (DP) as±60 V; collision energy as 35 V±15 eV; maximum candidate ions to monitor per cycle as 10; excluding isotopes within 4 Da.

References：

Ma Z, Yang K, Wang J, Ma J, Yao L, Si E, Li B, Ma X, Shang X, Meng Y, Wang H. Exogenous Melatonin Enhances the Low Phosphorus Tolerance of Barley Roots of Different Genotypes. Cells. 2023 May 16;12(10):1397. doi: 10.3390/cells12101397. PMID: 37408231; PMCID: PMC10217165.

HPLC

The column (C18, 4.6 × 250 mm, 5 μm) temperature was maintained at 35 ℃. An isocratic mobile phase consisted of methanol: deionized water: trifluoroacetic acid of 45:55:0.05 (v:v:v) and the flow rate was 0.8 ml/min . The injection volume was 10 μL.

References：

[1] A Y M , B P L , C C B W ,et al.Chlorine dioxide and sodium diacetate treatments in controlled atmospheres retard mold incidence and maintain quality of fresh walnuts during cold storage - ScienceDirect[J].Postharvest Biology and Technology, 161[2023-08-25].DOI:10.1016/j.postharvbio.2019.111063.

HPLC

Melatonin contents of nuts at harvest and for all treatments at 135 d were determined using a HPLC at 222 nm. The column (C18, 4.6×250 mm,5 μm ) temperature was maintained at 35℃. Anisocratic mobile phase consisted of methanol: deionized water: tri-fluoroacetic acid of 45:55:0.05 (v:v:v) and the flow rate was 0.8 ml/min. The injection volume was 10 μL.

References：

[1] A Y M , B P L , C C B W ,et al.Chlorine dioxide and sodium diacetate treatments in controlled atmospheres retard mold incidence and maintain quality of fresh walnuts during cold storage - ScienceDirect[J].Postharvest Biology and Technology, 161[2023-09-08].DOI:10.1016/j.postharvbio.2019.111063.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.