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CAS:18883-66-4
Appearance:White to light yellow powder
Storage:Store at -20℃，avoid light，2 years.
Purity:Min.75% as α-anomer
Description:
Effective DNA methylation reagent; Can produce diabetes in experimental animals, establish diabetes model
Instructions for use: STZ requires a pH4.2-4.5 (Cat: C1013) buffer configuration made of citric acid and sodium citrate.
Use and synthesis method:
Biochemical research. This product is a nitrosylurea antibiotic produced by Stre.achromogenes Uar.128, which is different from fat-soluble nitrosylurea in that it is a methyl group at the chloroethyl and an amino sugar at the other end of the molecule. STZ can decompose active methyl-carbon ions by itself and cross-link with DNA, so that DNA can be alkylated, but its alkylation effect is weaker than that of other nitrosylurea drugs, and the alkylation effect of its metabolite methylnitrosylurea is 3 to 4 times stronger than that of STZ. STZ forms isocyanates in the body. Thus, it binds to nucleic acid proteins, inhibits the activity of DNA polymerase, and makes it difficult to repair damaged DNA. In the course of anti-tumor studies, it was found that STZ can increase blood sugar in mice, and can cause diabetes in dogs and monkeys, and it is permanent. The diabetic effect of STZ is different in species and does not cause it in guinea pigs or humans. The mechanism of diabetes induced by STZ is mainly due to the decrease of nicotinamide adenine (DNA) content in islet cells. The glucose group in STZ molecules can make STZ enter the islet β cells, cause morphological changes in the β nucleus, and cause the chromosome agglutination, elongation and concentration.
Feature:
< p >features: purity: 98% or higher, or 75% of alpha anomer basis< br / >melting point: 121 ℃ (dec)< / p >
STZ molding precautions (reference) :
1. This product is prone to delixing and deactivation, it should be avoided from moisture, and it will fail after 30 minutes of moisture. Its aqueous solution is unstable, STZ requires drying and avoiding light, and dry aluminum foil (or tin foil) is recommended.
2. Configuration: Citric acid buffer dissolved STZ at 1-2% concentration, ice bath, freshly prepared before injection.
3, require rapid injection, easy to form high blood sugar, slow injection speed, low risk, but not easy to mold. Of course, the dose of STZ is a major factor in determining blood sugar levels.
4, citric acid buffer at the pH of 4.2-4.5 is more stable, too large or too small are easy to cause product inactivation.
If the model does not meet the standard, STZ can be supplemented three days later (intraperitoneally injected at a dose of 10mg-20mg/kg body weight), which is also easy to mold, or after the blood sugar is restored to normal, the conventional dose is injected; However, to achieve the ideal effect, it is often returned to the normal state to rebuild the mold.
Injection dose (Ref) :
Type I diabetes model: the rat dose was 65-70mg/kg; Or refer to product descriptions and literature.
Type 2 diabetes model: Rats fed high sugar and fat for 1-2 months with STZ doses of 25-40mg/kg or references.
Storage: -20℃
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
