T7 RNA Polymerase
Cat.No：R8570 Solarbio
Storage：-20℃
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PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
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Apoptosis
Metabolic Enzyme & Protease
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ER Stress
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DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
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Centrifuge
Shaker
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Product Information
| Storage | -20℃ |
| Note | 1. The reaction of in vitro transcription is highly sensitive to RNase, please not mix Rnase in the reaction system. Experimental equipment such as pipettes and EP tubes must be RNase-free. 2. Add samples with the order in the instruction strictly. 10×Transcription Buffer should be used after returning to room temperature. DNA is easy to co-precipitate with spermidine at low temperature, which affects the transcription yield. In the preparation of the system, DNA template should be the last component added. 3. For small DNA fragments (≤300bp) in vitro transcription, it is recommended to extend the vitro transcription time to 3 hours to ensure adequate yield. It will not affect the quality of the product if extend the vitro transcription time (in 16 hours). |
| Source | Recombinant E.coli |
| Unit Definition | 1U T7 RNA polymerase refers to the amount of enzyme required to incorporate 1nmol NTP into RNA at 37℃ and pH8.0 for 1 hour. |
| Concentration | 50U/ul |
| Solvent | 50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% (v/v) TritonX-100， 50%(v/v)Glycerol，pH7.9 at 25℃ |
| Unit | Piece |
| Specification | 5KU 10KU 50KU |
T7 RNA polymerase is a DNA-dependent RNA polymerase derived from bacteriophage T7 with highly specific 5'→3' end activity of RNA polymerase.T7 RNA polymerase has high specificity for the T7 promoter and is able to synthesize large amounts of RNA using the downstream sequence of the T7 promoter as a template.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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