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My Cart> Molecular Biology > PCR/qPCR > qPCR Kit > Blood And Saliva Direct qPCR Assay Kit
Storage：Storage at 4℃ for short time,? -20℃?for longer time.
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| Storage | Storage at 4℃ for short time,? -20℃?for longer time. |
| Note | 1. Sensi Direct Premix-UNG (Probe qPCR) is hot started for 1~5min. It is suitable for PCR reaction by multiple fluorescence quantitative probe method. 2. If the fluorescence value is too low or the amplification inhibition phenomenon is obvious, it is recommended to reduce the sample size or dilute the sample and then add the sample; It can also be improved by increasing the reaction volume (60 to 100 μL) while maintaining the same dosage. 3. The above blood, throat swab and other samples were collected in accordance with clinical standard operating requirements; To avoid nucleic acid degradation, freshly collected samples can be used. 4. The three-step method is recommended for primers with low annealing temperature or for amplification of long fragments over 200 bp. 5. Before and after amplification, please use special areas and pipettes, wear gloves to operate and change frequently; Do not open the reaction tube after the PCR reaction is completed to minimize the contamination of the PCR products to the sample. |
| Unit | Set |
| Specification | 100T |
Blood And Saliva Direct qPCR Assay Kit is a special reagent designed for direct detection of target gene fluorescence quantitative (probe) DNA amplification without the nucleic acid extraction and purification step of samples. This product has a strong inhibitor tolerance, and can be used for direct DNA amplification detection of anticoagulant whole blood, plasma, serum, throat swab, saliva and other samples without DNA extraction and purification.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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