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My Cart> Molecular Biology > Nucleic Acid Extraction/Purification > Extraction/Purification Auxiliary reagent > mRNA Capping Rate Pretreatment Kit
Storage：Storage mreagent, 10xM buffer and Buffer1 at -20℃, the remaining ingredients at 4℃. Do not freeze or thaw magnetic beads.
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| Storage | Storage mreagent, 10xM buffer and Buffer1 at -20℃, the remaining ingredients at 4℃. Do not freeze or thaw magnetic beads. |
| Note | 1. Save the components in the kit as required. MReagent, 10xMBuffer and Buffer1 are stored at -20℃, and the other reagents are stored at 4℃. Note that the adsorption magnetic beads can not freeze and thaw. 2. Magnetic beads, reagents and other materials provided in this kit have been screened and verified. In order to ensure the treatment effect, please do not change the materials provided in the kit at will, otherwise the experimental effect will not be guaranteed. 3. Step 3 is an optional step, which can reflect the effect of enzyme digestion treatment, and it is decided to continue to optimize the enzyme digestion conditions or recover the enzyme digestion products. In order to ensure the effect of on-machine testing, the processed samples should be tested by SDS-PAGE. 4. The samples treated by this kit should be tested on the machine in time, and the storage time of the samples should be less than 1 week as far as possible. The samples without caps stored for a long time are easy to degrade, which may lead to a higher cap rate than the actual test. Pay attention to personal protection during the experiment. |
| Unit | Set |
| Specification | 10T |
Capping rate is an important quality attribute index of mRNA vaccine drugs and a necessary validation data for the application of mRNA products. At present, the determination of cap rate is mainly carried out by LMS. There are hundreds of orders of magnitude difference in molecular weight between capped mRNA and uncapped mRNA, and it is difficult to accurately analyze the difference between capped and uncapped samples compared to the hundreds of thousands of molecular weight of intact mRNA. Therefore, analysis of the capping rate first requires processing long fragments of mRNA into small fragments, turning differences of the order of hundreds of thousands into differences of the order of thousands. The pre-treatment kit for mRNA capping rate detection provided by Solarbio can accurately and efficiently process mRNA samples, and the treated samples can be directly used for LMS analysis, helping to quickly analyze the sample capping rate. This kit is suitable for pretreatment of mRNA samples with different capping methods and various cap structures
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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