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Cell Acid Phosphatase Activity Assay Kit
Storage:2-8℃
Acid phosphatase ( ACP ) is widely present in various tissues, cells and body fluids. ACP catalyzes the hydrolysis of phosphate monoester to inorganic phosphate under acidic conditions, which is common in lysosomes of macrophages. ACP is often used in the auxiliary diagnosis of prostate cancer.
In an acidic environment, ACP catalyzes the hydrolysis of disodium phenyl phosphate to produce phenol, which reacts with 4-aminoantipyrine and potassium ferricyanide to produce red quinone derivatives with characteristic light absorption at 510 nm. ACP activity is calculated by measuring the increase rate of absorbance at 510 nm.
In an acidic environment, ACP catalyzes the hydrolysis of disodium phenyl phosphate to produce phenol, which reacts with 4-aminoantipyrine and potassium ferricyanide to produce red quinone derivatives with characteristic light absorption at 510 nm. ACP activity is calculated by measuring the increase rate of absorbance at 510 nm.
Remark：
These protocols are for reference only. Solarbio does not independently validate these
methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
