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Reactivity：Universal
Sample Type：Serum, plasma, Cell culture supernatant
Sensitivity：18.75 pg/ml
Storage：2-8℃，6 months
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| Name | F-TESTO ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | F-TESTO |
| Sample Type | Serum, plasma, Cell culture supernatant |
| Reactivity | Universal |
| Reaction Type | Competitive-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 31.25-2000 pg/ml |
| Sensitivity | 18.75 pg/ml |
| Specificity | This kit recognizes F-TESTO in samples. No significant cross_x005freactivity or interference between F-TESTO and analogues was observed |
| Precision | Coefficient of variation is < 10% |
| Recovery | 80%-120% |
| Assay Time | 2.5h |
| Detection Principle | Solarbio ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with F-TESTO. During the reaction, F-TESTO in samples or Standard competes with a fixed amount of F-TESTO on the solid phase supporter for sites on the Biotinylated Detection antibody specific to F-TESTO. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of F-TESTO in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Unit | Box |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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