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Reactivity：Human
Sample Type：Serum, plasma, Cell culture supernatant
Sensitivity：0.94 mIU/mL
Storage：2-8℃，6 months
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| Name | Human FSH ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | FSH |
| Sample Type | Serum, plasma, Cell culture supernatant |
| Reactivity | Human |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 1.56-100 mIU/mL |
| Sensitivity | 0.94 mIU/mL |
| Specificity | This kit recognizes Human FSH in samples. No significant cross-reactivity or interference between Human FSH and analogues was observed. |
| Precision | Coefficient of variation is < 10% |
| Recovery | 80%-120% |
| Assay Time | 3.5h |
| Detection Principle | This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FSH. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human FSH. You can calculate the concentration of Human FSH in the samples by comparing the OD of the samples to the standard curve. |
| Unit | Box |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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