Glutathione Mercaptotransferase (GST)

Product introduction

Glutathione S-transferase (GST, 2.5.1.18) is generally formed by the polymerization of two subunits between 25 and 27kDa in a homologous or heterologous manner. Its subunits have conserved serine/tyrosine (Ser/Tyr) residues at the N-terminus, and its hydroxyl group forms hydrogen bonds with the sulfhydryl group of glutathione (GSH), which is the specific binding site of GSH.

GST is a group of multifunctional isoenzymes widely distributed in various organisms. It is a group of enzymes related to the detoxification function of the liver. It mainly exists in the liver, and trace amounts exist in the kidney, small intestine, testis, ovary and other tissues. Increasing its hydrophobicity makes it easy to cross the cell membrane and expel it from the body after being broken down, thus achieving the purpose of detoxification. Because the liver is rich in GST in the cytoplasm, the enzyme is rapidly released into the blood when hepatocytes are damaged, resulting in an increase in serum GST activity.

Scope of application

GST is often used in the construction of efficient expression vectors in genetic engineering, so as to co-express with some difficult proteins as a molecular chaperone to achieve a soluble expression. In addition, GST antibody is often used to do Western Blot and other experiments to detect whether the gene is co-expressed, so the study of it is of great significance.

recommended name

EC 2.5.1.18

Isoelectric point

4.5

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Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

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