D-Mannose

Examples of using this product（for reference only）

HPGPC

CSPs (5.0 mg) was dissolved in 1.0 mL pure water and injected into the Shodex OH pak SB-804 column (300 mm × 8.0 mm i.d.). The flow rate of the mobile phase was 0.8 mL/min. The injection volume was 20 μL. T-series dextran was used as a standard.

References：

Jia Y, Xue Z, Wang Y, Lu Y, Li R, Li N, Wang Q, Zhang M, Chen H. Chemical structure and inhibition on α-glucosidase of polysaccharides from corn silk by fractional precipitation. Carbohydr Polym. 2021 Jan 15;252:117185. doi: 10.1016/j.carbpol.2020.117185. Epub 2020 Oct 2. PMID: 33183632.

IC

Ion chromatography was employed to determine the monosaccharide composition of the PCP-100, and it was coupled to a column (150 mm × 3 mm) and a pulsed amperometric detector.The PCP-100 (1 mg) was hydrolyzed by adding trifluoroacetic acid (2 M, 1 mL) for 3 h at 110 ℃. Following drying under nitrogen, the hydrolysate was mixed thoroughly with methanol to completely remove the excess trifluoroacetic acid. Subsequently, the hydrolysate was diluted with ultrapure water, and the injection volume was 1 mL. The mobile phase consisted of two solvents, in which mobile phase A was sodium hydroxide and mobile phase B was sodium acetate in sodium hydroxide. The flow rate was set at 0.4 mL/min and the column temperature was set at 30 ℃.

References：

Chen Y, Song L, Chen P, Liu H, Zhang X. Extraction, Rheological, and Physicochemical Properties of Water-Soluble Polysaccharides with Antioxidant Capacity from Penthorum chinense Pursh. Foods. 2023 Jun 10;12(12):2335. doi: 10.3390/foods12122335. PMID: 37372546; PMCID: PMC10297149.

GC

The six tea polysaccharides were hydrolyzed in 2 mol/L trifluoroacetic acid at 100 ℃ for 6 hr, respectively . The hydrolysate was reduced by NaBH4 and acetylation with acetic anhydride, converting into its respective alditol acetate. The derivative was analyzed by a GC (GC-7900, Shanghai, China) with a capillary column (HP-5, 30.0 m × 0.32 nm × 0.5 mm). The temperatures of injector and detector were 250 and 280 ℃, respectively . N2 was used as the carrier gas and maintained at 1.0 mL/min.

References：

Xu L, Chen Y, Chen Z, Gao X, Wang C, Panichayupakaranant P, Chen H. Ultrafiltration isolation, physicochemical characterization, and antidiabetic activities analysis of polysaccharides from green tea, oolong tea, and black tea. J Food Sci. 2020 Nov;85(11):4025-4032. doi: 10.1111/1750-3841.15485. Epub 2020 Oct 10. PMID: 33037621.

HPGPC

A Shimadzu LC-20AT chromatography unit equipped with a Shodex OH pak SB-804 column (8 × 300 mm i.d., YMC Co., Ltd, Kyoto, Japan) and a refractive index detector (Shimadzu RID-10 A) was employed in these studies.Briefly, pectin (5 mg) was dissolved in distilled water (1 mL) and injected into the column. Distilled water was used as the mobile phase, and the flow rate was 0.8 mL/min. The column temperature was maintained at 30 ℃. The injection volume was 20 μL.

References：

[1] A Y C , A Y W , A L X ,et al.Ultrasound-assisted modified pectin from unripe fruit pomace of raspberry ( Rubus chingii Hu): Structural characterization and antioxidant activities[J].LWT, 134[2023-10-11].

HPLC

HPLC analysis was performed on an LC20A HPLC system (Shimadzu, Japan) equipped with an SPD-20A ultraviolet detector and a C18 column (250 mm×4.6 mm, 5 μm, Shimadzu, Japan). The mobile phase was a mixture of 0.1 mol/L NaH2PO4-Na2HPO4 buffer (pH 6.7) and acetonitrile (83:17), and a flow rate of 1.0 mL/min was used. The wavelength of detection was 245 nm, and the column temperature was 30?C.

References：

Chen X, Sheng Z, Qiu S, Yang H, Jia J, Wang J, Jiang C. Purification, characterization and in vitro and in vivo immune enhancement of polysaccharides from mulberry leaves. PLoS One. 2019 Jan 2;14(1):e0208611. doi: 10.1371/journal.pone.0208611. PMID: 30601811; PMCID:PMC6314569.

HPLC

Chromatographic conditions as followed: Zorbax Eclipse Plus-C18 (4.6 mm × 100 mm, 5 μm, Agilent, USA) capillary column, ultraviolet detector (250 nm detection wavelength), mobile phase consisting of 0.025 M phosphate buffer (pH 6.7) with 83% (solution A) and 17% acetonitrile (solution B) in water, 0.5 mL/min flow rate and column at 30 ℃.

References：

Yang B, Wu Q, Luo Y, Yang Q, Wei X, Kan J. High-pressure ultrasonic-assisted extraction of polysaccharides from Hovenia dulcis: Extraction, structure, antioxidant activity and hypoglycemic. Int J Biol Macromol. 2019 Sep 15;137:676-687. doi: 10.1016/j.ijbiomac.2019.07.034. Epub 2019 Jul 5. PMID: 31284006.

HPLC

4 μL of the filtrate was analyzed using an Agilent 1260 Series HPLC system (Agilent Technologies, CA, USA). Chromatographic conditions were as follows: Zorbax Eclipse Plus-C18 (4.6 mm × 100 mm, 5 μm, Agilent, USA) capillary column, ultraviolet detector (250 nm detection wavelength), mobile phase consisting of 0.025 M phosphate buffer (pH 6.7) with 83% (solution A) and 17% acetonitrile (solution B) in water, 0.5 mL/min flow rate and column temperature at 30 ℃.

References：

Yang B, Wu Q, Luo Y, Yang Q, Chen G, Wei X, Kan J. Japanese grape (Hovenia dulcis) polysaccharides: New insight into extraction, characterization, rheological properties, and bioactivities. Int J Biol Macromol. 2019 Aug 1;134:631-644. doi: 10.1016/j.ijbiomac.2019.05.079. Epub 2019 May 15. PMID: 31100397.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.