Molecular Weignt Standard of Dextran T10

Due to differences between batches, molecular weight data is for reference only.Examples of using this product（for reference only）

HPGPC

High performance gel permeation chromatography (HPGPC) was applied in the determination of the homogeneity and average molecular weight of the PCP-100, which was combined with a refractive index detector (RID) and a column (7.8 mm × 300 mm). After filtration through a 0.22 μm membrane, 20 μL of PCP-100 solution (0.8 mg/mL) was injected into the HPGPC system and carried out with ultrapure water at flow rate of 0.6 mL/min. The average molecular weight was calculated from a calibration curve, which was constructed by reference to T-series dextran standards (T10, T40, T70, T500, and T2000).

References：

Chen Y, Song L, Chen P, Liu H, Zhang X. Extraction, Rheological, and Physicochemical Properties of Water-Soluble Polysaccharides with Antioxidant Capacity from Penthorum chinense Pursh. Foods. 2023 Jun 10;12(12):2335. doi: 10.3390/foods12122335. PMID: 37372546; PMCID: PMC10297149.

HPGPC

CSPs (5.0 mg) was dissolved in 1.0 mL pure water and injected into the Shodex OH pak SB-804 column (300 mm × 8.0 mm i.d.). The flow rate of the mobile phase was 0.8 mL/min. The injection volume was 20 μL. T-series dextran was used as a standard.

References：

Jia Y, Xue Z, Wang Y, Lu Y, Li R, Li N, Wang Q, Zhang M, Chen H. Chemical structure and inhibition on α-glucosidase of polysaccharides from corn silk by fractional precipitation. Carbohydr Polym. 2021 Jan 15;252:117185. doi: 10.1016/j.carbpol.2020.117185. Epub 2020 Oct 2. PMID: 33183632.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.