CAS:9004-54-0
Appearance:Off-white Powder
Storage:RT.
Purity:HPLC≥98%
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HPGPC
High performance gel permeation chromatography (HPGPC) was applied in the determination of the homogeneity and average molecular weight of the PCP-100, which was combined with a refractive index detector (RID) and a column (7.8 mm × 300 mm). After filtration through a 0.22 μm membrane, 20 μL of PCP-100 solution (0.8 mg/mL) was injected into the HPGPC system and carried out with ultrapure water at flow rate of 0.6 mL/min. The average molecular weight was calculated from a calibration curve, which was constructed by reference to T-series dextran standards (T10, T40, T70, T500, and T2000).
References:
Chen Y, Song L, Chen P, Liu H, Zhang X. Extraction, Rheological, and Physicochemical Properties of Water-Soluble Polysaccharides with Antioxidant Capacity from Penthorum chinense Pursh. Foods. 2023 Jun 10;12(12):2335. doi: 10.3390/foods12122335. PMID: 37372546; PMCID: PMC10297149.
HPGPC
CSPs (5.0 mg) was dissolved in 1.0 mL pure water and injected into the Shodex OH pak SB-804 column (300 mm × 8.0 mm i.d.). The flow rate of the mobile phase was 0.8 mL/min. The injection volume was 20 μL. T-series dextran was used as a standard.
References:
Jia Y, Xue Z, Wang Y, Lu Y, Li R, Li N, Wang Q, Zhang M, Chen H. Chemical structure and inhibition on α-glucosidase of polysaccharides from corn silk by fractional precipitation. Carbohydr Polym. 2021 Jan 15;252:117185. doi: 10.1016/j.carbpol.2020.117185. Epub 2020 Oct 2. PMID: 33183632.
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